Starfish Coelomic fluids: A novel promising trigger of wound healing of the skin in both diabetic and burned BALB/c mice models concomitant with hydrogel and supervised their wound closures using mask R-CNN software.

Summary

Recently, natural bioactive materials were extracted and separated from a certain type of starfish in China. Moreover, such extracted bioactive materials proven to form excellent pool of molecules for regeneration therapy.

Tissue regeneration is an important phenomenon with wide biological implications throughout the animal kingdom.

In this proposal two animal models will be tested to evaluate the capability and efficacy of the starfish coelomic fluids in wound heling/Tissue regeneration. The starfish coelomic fluid will be extracted and collected after the amputation of portions of the arm of Astropectin sp. (starfish). BAlB/c mice will be used in this study after the induction of diabetes type I using streptozotocin, and after being burned by a stamp of well-known diameter to estimate the degree of wound heling/Tissue regeneration after the application of the starfish coelomic fluids after being compared with negative and positive control groups. The study is designed to observe the wound heling/Tissue regeneration histologically, immunohistochemically, ultrastructurally, molecularly, and immunologically. Also, the wounds/burns will be photographed by digital camera for Mask R-CNN (R101FA Backbone network) to be furtherly-analyzed by certain software and compare the obtained results from each model in addition to the comparison of the results obtained from the groups /each model.

Side by side to the aforementioned used parameters on the two animal models, starfish will be collected, Classified, counted, traced and mapped by using geospatial technology (remote sensing, GIS and GPS) on certain localities along the coastal shore of the Red Sea, Egypt.

Collectively, from all of the above, the natural bioactive materials extracted from Astropecten sp. After amputation of its arm will be sprayed/carried on hydrogel and used as newly-synthesized tissue bandage that may accelerate the wound heling/Tissue regeneration depending on two main factors: firstly: the capability of hydrogel to absorb tissue fluids exudates from the wounded/burned skin of both mice models; so, the skin will be dried faster and be able to absorb the sprayed or applied extracted starfish coelomic fluids. Secondly: the capability of starfish coelomic fluids ingredients to accelerate the wound healing/skin regeneration. 

Finally, the resulting synthesized medical bandage will be highly valuable, because it will be manufactured from two natural resources (hydrogel, extracted coelomic fluid materials) as there is an increase of interest of natural products in modern medicine as part of disease management in the interest of providing the best patient care and minimizing cost. 

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