New Prototype containing Nano Optical Sensor for Follow up the Liver Cancer Disease During and After Treatment of Patient

Summary

This proposal aims to preparation and research studies of a thin film doped the nano optical sensor that is used to monitor and diagnose liver cancer during and after treatment for liver cancer patients. Diagnosis is the first step in treating the disease. If the diagnosis is made in a precise and sensitive manner. the diagnosis of disease will be detected more easy.  Alpha fetoprotein is used as a biomarker in the human body to identify liver cancer. The concentration and number of the Alpha fetoprotein units in blood sample of liver cancer patients are determined by PCR, chemiluminescence or ELISA techniques. These techniques require considerable time to prepare the sample for measurement and expensive chemicals that are constantly purchased, for obtaining the results of test. this test takes between a day and a few days and requires the patient to go to the laboratory to take the sample, which leads to exhaustion of the patient and the time. All of these problems that are met by the chemist based on the measurement conditions or the patient who needs to provide physical effort will be overcome after the use of the prepared thin films doped the nano optical sensor which will be used in the second stage to manufacture a new portable mobile ready to use form prototype manufactured locally, which will be based on the analysis of the patient's blood sample in the patient`s home. The analysis will be carried out by put one drop of the patient`s blood on a thin slit has the ability to select the Alpha fetoprotein from the patient’s blood. The number of Alpha fetoprotein units is determined by interaction of the slit containing the Alpha fetoprotein with the light emitted from the film doped the nano optical sensor in the locally fabricated prototype then translation of the light exist from the optical sensor after interaction with slit of Alpha fetoprotein to electrical signal then numerical digits on the readout of the prototype, which it can be read easily. This prototype will replace the PCR and Eliza techniques in the future.

Achievements

all stages are achieved :

Stage 1: Evaluate Feasibility  

Task 01: Conduct feasibility studies and preliminary survey structure.

Task 02: Estimated the overall performance and cost.

Task 03: Selecting the proper nano optical sensor.

Task04: Revise stage 2 and 3 plans based on stage 1 investigation

Task05: Stage 1 program management and reporting

Stage 2: Questionnaire Preparation 

Task 06: Starting synthesis of sensors (Ln3+; Sm, Tb and Eu complexes or Pd complexes and Pt complexes) thin film /silica nanooptode.

Task 07:  Conducting characterization of the prepared nano optical sensors in order to have a large surface area-to-volume ratio and a selectivity and sensitivity.

Task 08: Starting evaluation of the optimum conditions the nano optical sensor to obtain a high selectivity and sensitivity for the analyte”.

Task 09: sensor calibration to the liver cancer assessment

Task 10: sensor validation to different liver diseases of patient samples.

List of Publications from the Project

1-Photophysical properties of some transition metal complexes in different media and testing their ability as sensor for determination of Alpha Fetoprotein

2- Highly selective determination of Alpha Fetoprotein in different real samples of liver cancer patients based on transition metal complex embedded in a thin film polymer

Partners

Project Members

Project Leaders

• Mohamed Said Attia